Foot and mouth disease (FMD) is the most contagious disease of mammals and has a great potential for causing severe economic loss in susceptible cloven-hoofed animals. There are seven serotypes of FMD virus (FMDV), namely, O, A, C, SAT 1, SAT 2, SAT 3 and Asia 1.The VDx® FMDV qRT-PCR product line is a reagent for highly sensitive detection of FMDV in infected samples. This reagent is composed of 1-step premix, which can be directly added to the premix to prevent cross-contamination in the laboratory, and it is possible to check the reaction in real time. For reactions, simply add template (DNA). The test reliability can be secured by Control DNA for Validation. Depending on the purpose, differential diagnosis of major serotypes is possible using Type O, A, and Asia 1 qRT-PCR.